Proteolysis of myosin during platelet storage.

نویسندگان

  • J Abramowitz
  • A Stracher
  • T C Detwiler
چکیده

Physical properties of actomyosin from either fresh or stored platelets have been compared. Actomyosin obtained from platelets after 3 days of storage contained myosin that was 60-80% degraded to myosin rod. No myosin rod was detected in fresh platelets. The platelet myosin rod is similar to the rod produced by limited proteolysis of skeletal muscle myosin.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage

Abstract Background and Objectives Platelets release microparticles containing cellular components, including microRNAs, during storage. Assessment of these microRNAs is one of the markers for evaluation of platelet storage lesions. The aim of the present study was to evaluate the level of changes in the expression of mir-223, mir-92a and mir-222 during storage in platelets prepared by platele...

متن کامل

Platelet Factor 3 Based-clotting Time Assay as a Quality Marker for Long-term Storage of Platelet Concentrates

Background: Platelets rapidly lose their qualities usually after 5 day of storage. Different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. The purpose of this study was to demonstrate if platelet factor 3 (PF3) assay could be an indicator of storage lesion and provide ...

متن کامل

Flow Cytometric Measurement of CD41/CD61 and CD42b Platelet Receptors and Clotting Assay of Platelet Factor 3 During Long Term-Storage of Platelet Concentrates

Background: The purpose of the present in vitro study was to evaluate the effect of long term storage of conventional platelet concentrates (PCs) on major platelet receptors CD42b and CD41/CD61 by flow cytometry method and also measuring the overall platelet procoagulant activity status using platelet factor 3 (PF3) assay. Materials and Methods: Six random units of conventional platelet conce...

متن کامل

Effect of Different Somatic Cell Levels on Nitrogen Components of Yoghurt Milk and Probiotic Set Yoghurt During Storage Life

Nitrogen components of yoghurt milk samples and resulting probiotic set yoghurt weredetermined. Analyses included total nitrogen (TN), non-casein nitrogen (NCN), non-proteinnitrogen (NPN), casein nitrogen (CN) and proteolysis index (PI). Yoghurt samples wereanalyzed 1, 7, 14 and 21days after production and storage at 5±1°C. TN, CN and PI contentsof yoghurt milk samples with high Somatic Cell SC...

متن کامل

An Overview on Platelet-derived Microparticles in Platelet Concentrates: blood collection, method preparation and storage

Preparations of platelet concentrates (PCs) that are stored under blood bank conditions and used for transfusion purposes, appear to be enriched in platelet derived-microparticles (PMPs) with high coagulant activity that may change platelet efficacy and safety issues. High shear stress could cause shedding of PMPs from the platelet plasma membrane, platelet aggregation, and activation of the co...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of clinical investigation

دوره 53 5  شماره 

صفحات  -

تاریخ انتشار 1974